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. 2012 Jan 17;7(1):e29242. doi: 10.1371/journal.pone.0029242

Figure 3. Schematic diagram of PCR-RFLP strategy for detecting L1014F and L1014C substitutions and predicting the size of PCR-RFLP products in the para sodium channel gene in An. sinensis.

Figure 3

A: A“A”to “G” substitution at the EP1-primer causes the formation of a diagnostic HindIII recognition site (gray shade) at position 42-bp of the PCR fragment.“M” is a marker. The substitution can be inferred from the appearance of the agarose gel electrophoresis: “1,2,3,5,7,9” are (T/T), “4,6” are (T/G), “8” is (G/G). B:A“C”to “T” substitution at the EP4-primer causes the formation of a diagnostic HphI recognition site (gray shade) at position 32-bp of the PCR fragment.“M” is a marker. The substitution can be inferred from the appearance of the agarose gel electrophoresis: “2,3,4,6,7,8,9” are (T/T), “1,5” are (T/G).