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. 2012 Jan 17;7(1):e28936. doi: 10.1371/journal.pone.0028936

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Puffenberger et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Wild-type N-terminal FLAG-HARS localizes to the cytoplasm. (B) Mutant N-terminal FLAG-HARS (p.Tyr454Ser) localizes to the cytoplasm in a manner that is indistinguishable from the wild-type localization shown in A. Transfected and non-transfected cells were labeled with anti-FLAG M2 monoclonal antibody and AlexaFluor 488-conjugated anti-mouse IgG₁ (green fluorescence). (C) Reaction velocity vs. human tRNA^His concentration for histidine aminoacylation of tRNA^His by wild-type murine HARS (HARS) and p.Tyr454Ser (Y454S) HARS. Scale bar = 10 µm in A and B.