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. 2012 Jan 17;7(1):e30225. doi: 10.1371/journal.pone.0030225

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(A) Graph of A_max/EC₅₀ vs. TIF2. The results of one representative experiment (n = 4), conducted as in Fig. 1, are shown for the indicated amounts of transfected TIF2 plasmid, corrected for non-linear protein expression. (B). Determination of intersection coordinates. The coefficients “a” and “b” of the linear plots in A, described by y = a+bx, are graphed. The negative value of the slope, and y-axis intercept, in this graph give the x- and y-axis the values respectively for intersection point of the lines in A, as described in the text and Text S1. Data for one representative graph each (n = 4) of 1/EC₅₀ vs. TIF2 (C) or sSMRT (D), of A_max/EC₅₀ vs. sSMRT (E) and of EC₅₀/A_max vs. sSMRT (F) were acquired as in A and plotted against the indicated amounts of transfected plasmids, each of which have been corrected for non-linear protein expression.