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. 2012 Jan 17;7(1):e30225. doi: 10.1371/journal.pone.0030225

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Cos-7 or 293 cells were transiently transfected with the same amount of plasmids for glucocorticoid receptor (0.5 ng), NCoR (90 ng) or equimolar amount of control plasmid (51.6 ng of human serum albumin in the pCMX vector), and GREtkLUC reporter and induced with EtOH ±1 µM Dex as described in Materials and Methods. The total Luciferase activity with 1 µM Dex with each treatment was expressed relative to that with no added NCoR (± S.D., n = 3). P values: *≤0.021.