Table 1. Algorithms for single factor plots for factor F.

Plot parameters	Plot properties	Mechanistic conclusions
1/EC50 vs. F	linear with zero slope (i.e. does not change with F)	1) F = A at CLS or 2) F = PN before CLS
	linear with positive slope	1) F = A not at CLS; or 2) F = U before or at CLS, or 3) F = PU before CLS
	nonlinear decreasing curve (concave-up)	1) F = C, 2) F = M before or at CLS
	nonlinear increasing curve (concave-down)	1) F = LM or PM, before CLS; or 2) F = M at CLS
Amax/EC50 vs. F	linear; y-axis intercept = 0	1) F = A before or at CLS
	linear; y-axis intercept >0	1) F = A after CLS; or 2) F = PU before or at CLS
	nonlinear decreasing curve that approaches zero for large F	F = C before or at CLS
	nonlinear decreasing curve that approaches positive value for large F	1) F = PM or PN, before or at CLS; or 2) F = C after CLS
	nonlinear increasing curve	1) F = PM or PN, before or at CLS
EC50/Amax vs. F	linear with positive slope	1) F = C before or at CLS