Table 6. Primers-set sequences used to analyze gene expression by quantitative PCR.
Gene | Forward primera | Reverse primera | Amplicon length (bp) | PubMed Accession No. |
PEPCK | CTGCTGGTGTGCCTCTTGTA | TTCCCTTGGCTGTCTTTCC | 259 | NM_205471 |
ACLY | GGCGTGAATGAACTGGCTAAC | TAGTCTTGGCATAGTCATAGGTCTGTTG | 79 | NM_001030540 |
ME | TGCCAGCATTACGGTTTAGC | CCATTCCATAACAGCCAAGGTC | 175 | NM_204303 |
FAS | TGAAGGACCTTATCGCATTGC | GCATGGGAAGCATTTTGTTGT | 96 | NM_205155 |
GAPDH | TGCCATCACAGCCACACAGAAG | ACTTTCCCCACAGCCTTAGCAG | 123 | NM_204305 |
Beta-2 microglobulin | AAGGAGCCGCAGGTCTA | CTTGCTCTTTGCCGTCATAC | 151 | Z48921 |
Sequences are indicated from 5′ end to 3′ end of oligonucleotides.