Figure 4. VU0183254 reduces Orco-mediated activity in vivo.

A. Representative traces of Cp neuron activity in response to vehicle (DMSO) or VU0183254 as measured by single-sensillum electrophysiology. Activity was allowed to stabilize for 10 seconds and then recorded for 60 seconds. CpA spikes can be distinguished by their larger amplitude in expansions (right panel). CpB/C spikes (counts below traces) are reduced in the presence of VU0183254. B. Spontaneous CpA spike rates are unaffected by the presence of VU0183254 (n = 8). Spikes were counted for each of the first 10 seconds and then averaged across the remaining 10-second intervals. Normal CpA activity is confirmed by CO₂ pulse delivered at the end of the recording period. C. Spontaneous firing rates of CpB/C neurons are reduced by VU0183254 in a dose-dependent manner (n = 8). All spikes were distinguished and quantified using AutoSpike software (Syntech).