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. 2012 Jan 17;7(1):e30311. doi: 10.1371/journal.pone.0030311

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Wei et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Endogenous PDCD4 in both OV2008 and C13 ovarian cancer cells was localized exclusively in the nucleus when cultured in medium with serum. More cytoplasmic localization of PDCD4 was observed under serum starvation treatment. Endogenous PDCD4 was detected by immunofluorescent staining using PDCD4 primary antibody and FITC-labeled goat anti-rabbit secondary antibodies. Dapi staining indicated nuclear localization. Representative translocation was indicated by arrows.