Figure 2. Identification of the downstream target genes of EZH2 associated with metastasis of prostate cancer.

(A) RT² profiler PCR array for Human Tumor Metastasis genes in DU145 cells after an infection with EZH2-specific shRNA or control (NT) lentivirus. The scatter plot of the test vs control samples indicates the validity of the experiment. (B) 12 genes whose expressions are highly affected by EZH2 knockdown are shown (also see Tables S1 and S2). Total RNA was isolated from DU145 cells after an infection with EZH2-specific shRNA or control (NT) lentivirus and processed for PCR array (C) Immunofluorescence images of normal prostate (Normal 1 and Normal 2) and prostate tumor tissue sections (PCa1 and PCa2), using anti-EZH2 antibody (green) and anti-TIMP3 antibody (red). Nuclei were stained with DAPI (blue).