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(A) Western blot analysis of endogenous levels of EZH2, TIMP3 and 3meH3K37 in the prostate cancer cells (PC3 cell) after treatment with DZNep (5 µM) or DMSO (control) for 2 days. Actin was used as an internal control. (B) qRT–PCR analysis of expression of TIMP3 in PC3 cells treated with DZNep (5 µM) or DMSO (control). (C) Invasion assay of PC3 cells treated with DZNep (5 µM) or DMSO (control).
