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Cells were embedded and lyzed in agarose and their chromosomes were electrophoresed and stained with ethidium bromide. A. 600–1300 kb electrophoresis B. 100–500 kb electrophoresis. The field isolates 017102, 431462, 072218 and 18693 from Afghanistan have closely related karyotype to the L. tropica reference strains 175 and MHOM/SU/74/K27. M, yeast chromosomes molecular weight marker (BioRad).
