Table 3.
icaA expression values using RNA extracted from the three different kits
| Extraction kit | Strain | 16S rRNA Ct | icaA Ct | icaA expression |
|---|---|---|---|---|
| FastRNA® | 1457 | 17.36 ± 0.41 | 31.48 ± 0.28 | 5.62 × 10-5 |
| M187 | 12.27 ± 0.81 | 27.07 ± 1.05 | 3.48 × 10-5 | |
| PureZOL™ | 1457 | 17.99 ± 0.30 | 31.84 ± 0.11 | 6.77 × 10-5 |
| M187 | 15.71 ± 0.83 | 29.58 ± 1.94 | 6.20 × 10-5 | |
| PureLink™ | 1457 | 15.25 ± 0.12 | 32.56 ± 0.11 | 0.62 × 10-5 ** |
| M187 | 13.83 ± 0.67 | 31.13 ± 4.02 | 0.62 × 10-5 ** | |
Total RNA (40 ng) was used to synthesize cDNA for qPCR. The icaA expression was calculated by the delta Ct method (2ΔCt). The values represent the mean plus or minus standard deviation of 2 to 4 independent experiments (**P < 0.01).