| E. coli |
|
|
| CC118 (pir) |
(ara-leu) araD lacX74 galE galK phoA20 thi-l rpsE rpoB argE(Am) recAl pir |
48 |
| SM10 (pir) |
thi-l thr leu tonA lacY supE recA::RP4-2-Tcr::Mu, Kmr, pir |
48 |
| P. luteoviolacea |
|
|
| HI1 |
Wild-type isolate |
12 |
| ATCC3349 |
Isolated from seawater, France, ATCC number: 33492 |
26 |
| HI1, Strr
|
Spontaneous streptomycin-resistant, mutant of Hawaii strain |
This study |
| Plasmids |
|
|
| pLOF/Km |
Ampr (Tn10-based delivery plasmid, Kmr) |
48 |
| pCVD442 |
Apr; Sucroses, 1.2-kb SacB gene inserted into pGP704 at cloning site PstI |
41 |
| pCVD443 |
Apr, Kmr, Sucroses, pCVD442 with Kmr gene inserted at SmaI site |
This study |
| pDM 1 |
Apr, Kmr, Sucroses, pCVD443 with 1.6kb of DNA flanking ORF1 |
This study |
| pDM2 |
Apr, Kmr, Sucroses, pCVD443 with 1.6kb of DNA flanking ORF2 |
This study |
| pDM3 |
Apr, Kmr, Sucroses, pCVD443 with 1.6kb of DNA flanking ORF3 |
This study |
| pDM4 |
Apr, Kmr, Sucroses, pCVD443 with 1.6kb of DNA flanking ORF4 |
This study |
| pDM5 |
Apr, Kmr, Sucroses, pCVD443 with 1.6kb of DNA flanking ORF5 |
This study |
| pDM6 |
Apr, Kmr, Sucroses, pCVD443 with 1.6kb of DNA flanking ORF6 |
This study |
| pDM7 |
Apr, Kmr, Sucroses, pCVD443 with 1.6kb of DNA flanking ORF7 |
This study |
