Table 3. Sequences of oligonucleotides used in genome walking.
Adaptor 1 | 5-CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGCA GGT-3 |
Adaptor 2 | 3-H2N-CCCGTCCA-P-5 |
Adaptor 1 primer | 5-GATCCTAATACGACTCACTATAGGGC-3 |
Adaptor 1 nested primer | 5-A ATA GGG CTC GAG CGG C- 3 |
Tn10C | 5-GCTGACTTGACGGGACGGCG-3 |
Tn10D | 5-CCTCGAGCAAGACGTTTCCCG-3 |