Table 1.
Primer sets (5′-3′) used for real-time PCR analysis of gene expression
Gene of interest | Forward primer | Reverse primer |
---|---|---|
Cystic fibrosis | tgttgtgaagacgagctgatg | tgcattaaaagagagcatgtgttg |
Nicotinamide adenine dinucleotide dehydrogenase-5 | tggatgatggtacggacgaa | tgcggttatagaggattgcttgt |
Complex I (ND1) mitochondrially-encoded | caggatgagcctcaaactcc | ggtcaggctggcagaagtaa |
Complex I (ND2) | agggatcccactgcacatag | cctatgtgggcaattgatga |
Complex II (SDHC) nuclear-encoded | acaaatggtctcttcctatggca | cccctccactcaaggctattc |
Complex III (cytb) nuclear-encoded | acgtccttccatgaggacaa | gaggtgaacgattgctaggg |
Complex IV (cox1a) mitochondrially-encoded | cttttatcctcccaggatttgg | gctaaatactttgacaccgg |
Complex IV (cox5a) nuclear-encoded | ctttaaatgaattgggaatctccac | gcccatcgaagggagtttaca |
Complex V (atp6) | aattacaggcttccgacacaaac | tggaattagtgaaattggagttcct |
Peroxisome proliferator-activated receptor γ coactivator 1 alpha | caatgaatgcagcggtctta | gtgtgaggagggtcatcgtt |
Insulin-like growth factor 1 | ctgagctggtggatgctctt | cactcatccacaatgcctgt |
Adenine nucleotide transporter 1 | acttcgccttcaaagacaagtaca | gcgccagaactgcttatgg |
β2-microglobulin | aagtatactcacgccaccca | aagaccagtccttgctgaag |