Fig. 2.
Hydroxytyrosol extends chronological lifespan after long duration of quiescence. Sixty-day control and HT-treated quiescent cultures of NHFs were replated at a lower cell density and cell growth characteristics were measured by flow cytometry analysis of BrdU-labeled cells at 48-h post-plating: a representative BrdU vs. PI density plot of DNA content; b percentage of S and G2 phases were evaluated using FlowJo and ModFit software; c cell numbers at 2- and 4-day post-plating. Asterisks indicate significant difference compared with untreated control. n = 3; p < 0.05