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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Arch Oral Biol. 2011 Sep 16;57(2):197–204. doi: 10.1016/j.archoralbio.2011.08.013

Figure 1.

Figure 1

Western blot analysis of rhSPLUNC1 and immunoprecipitated rhSPLUNC1 to show that the capture and detection antibodies both recognized rhSPLUNC1. (A) Western blot showing rhSPLUNC (lane 4); rhSPLUNC1 immunoprecipitated with mouse IgG2b anti-rhSPLUNC1 monoclonal antibody (MAB1897 (lane 6); and rhSPLUNC1 immunoprecipitated with goat anti-rhSPLUNC1 antibody (AF1897) (lane 7) and probed with AF1897. (B) Western blot showing rhSPLUNC (lane 12); rhSPLUNC1 immunoprecipitated with mouse IgG2b anti-rhSPLUNC1 monoclonal antibody (MAB1897) (lane 14); and rhSPLUNC1 immunoprecipitated with AF1897 (lane 15) and probed with mouse IgG2b anti-rhSPLUNC1 monoclonal antibody (MAB1897). Both blots had nearly identical profiles suggesting that both antibodies recognized rhSPLUNC1.