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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Arch Oral Biol. 2011 Sep 16;57(2):197–204. doi: 10.1016/j.archoralbio.2011.08.013

Figure 2.

Figure 2

Two-Dimensional Fluorescence Difference Gel Electrophoresis (2-D DIGE) gels comparing rhSPLUNC1 (A) with SPLUNC1 immunoprecipitated from a saliva sample with commercial goat anti-rhSPLUNC1 antibody (AF1897) (B) and mouse IgG2b anti-rhSPLUNC1 monoclonal antibody (MAB1897) (C). rhSPLUNC1 and immunoprecipitated SPLUNC1 were labeled with dyes, separated in a pH 3–10 linear range in the first dimension, and separated in 10.5% gel in the second dimension. Nearly identical 2-D DIGE profiles were seen among rhSPLUNC1, rhSPLUNC1 immunoprecipitated with AF1897, and rhSPLUNC1 immunoprecipitated with MAB1897 suggesting that both antibodies recognized SPLUNC1 in saliva. The relative location of SPLUNC1 is shown. Other spots represent a carry-over of protein from the immunoprecipitation process. Trace contaminants like albumin, salivary alpha amylase, MMP27, immunoglobulin kappa light chain variable region, etc were all detected.