Figure 2.

Toluene and ethanol effects on coordination and body bends made during locomotion. Representative images of individual wild-type young-adult worms during an 8-second period of forward locomotion. Each column shows images highlighting the position of one animal approximately 1 second apart. Column 3 is an untreated animal crawling on agar media. Column 1 and 2 show animals that have been exposed to 10,000 ppm and 2,000 ppm toluene, respectively, and imaged immediately after removal from the static vapor chamber. Columns 4 and 5 are animals still in the presence of ethanol (200 mM and 400 mM, respectively) and have been exposed for 10 minutes prior to imaging. The worms have been centered in each cropped image, reference points have been marked with symbols (×, *, +) to allow relative movement of the worms to be judged. Scale bar equals 250 μm.