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Pretreatment of rat cortical neuron cultures with pertussis toxin (PTX; 18 h, 100 ng/ml) abolished the inhibitory action of orexin A (1 μM) on forskolin (1 μM) and PACAP27 (0.1 μM) induced increase in cyclic AMP formation. Values shown are means ± SEM (n = 9–12). a P < 0.05 versus control, b P < 0.05 versus Forskolin or PACAP27, c P < 0.05 versus Forskolin or PACAP27 and orexin A
