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RNA-FISH of an X-borne gene (red) and an autosomal control (green) in male and female fibroblasts of three eutherian species. Transcription from the two autosomal alleles (green) is detected in all nuclei. a Male fibroblast nuclei show transcription from the single X-borne allele, whereas female fibroblast nuclei show transcription from either one (b) or two (c) X-borne ATRX alleles. Nuclei are counterstained with DAPI (blue). HSA Homo sapiens, LAF Loxodonta africana, MMU Mus musculus
