FIG. 5.
Transfection efficiency in LPB (A), SA-1 (B), EAT (C), and B16 (D) tumors after pretreatment of tumors with collagenase and hyaluronidase. EP Luciferase, injection of plasmid DNA followed by application of EP; Collagenase+EP Luciferase, injection of collagenase 24 hr before electrotransfection of tumors; Hyaluronidase+EP Luciferase, injection of hyaluronidase 2 hr before electrotransfection of tumors; Combination+EP Luciferase, injection of collagenase 24 hr and hyaluronidase 2 hr before electrotransfection of tumors. Data represent the mean±SEM of 3 or 4 tumors from 3 independent experiments (total, 9–12). *p<0.05 compared with other treatment groups; #p<0.05 compared with the Hyaluronidase+EP Luciferase group.