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A model for rAAV packaging. The 3′ end of AAV ITR is inserted into the preformed capsid first. a, early termination signal (ETS) leading to DI particles; b, mature termination signal (MTS), which is the 5′ ITR; c, maximal capacity of the vector; Rep78 and Rep52, viral proteins involved in rAAV packaging; *, terminal resolution site of the ITR.
