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Schematic view of the lentiviral vector and of the SFFV promoter–transgene sites susceptible to CpG methylation. The upper panel shows SIN HIV-1-based viruses, encoding the SFFV-LTR promoter, the HIV-1 cPPT element, and a mutated WPRE. Following codon optimization, multiple novel CpG sites were introduced throughout the SPINK5co transgene compared with the native SPINK5 gene (MethylPrimer Express Software v1; Applied Biosystems, Foster City, CA).
