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. 2011 Sep 6;23(1):83–90. doi: 10.1089/hum.2011.091

FIG. 2.

FIG. 2.

SFFV promoter in combination with SPINK5co results in methylation-mediated DNA silencing. NTERT keratinocytes transduced with lentiviral vectors encoding SFFV-GFP (A) or SFFV-SP/GFP (B) were cultured in the presence or absence of 5 μM azacitidine (aza) for a period of 40 days. GFP intensity in transduced cells cultured over time was monitored by flow cytometry. (A) GFP intensity in cells transduced with SFFV-GFP remained stable, and azacitidine treatment had no significant effect on these cells. (B) GFP intensity in cells transduced with SFFV-SP/GFP decreased over time and was rescued by azacitidine treatment. (C) LEKTI expression in NTERT cells transduced with SFFV-SP/GFP detected by western blot decreased over time (lanes 2–5) and were rescued by azacitidine treatment (lanes 6–9).