FIG. 3.
Growth rate and cell-killing assays for wild-type and recombinant viruses. (A) Viral growth rates after infection of 293Her2/neu cells. Cells were infected with 20 PFU/cell for 1 hr at 37°C and harvested 24, 48, and 72 hr postinfection. The number of plaque-forming units per cell was determined by end-point dilution. Symbols: AdΔE1[WT] (open circles), AdΔE1[ZH/1] (open triangles), AdΔE1[ZH/2] (solid squares), and AdΔE1[ZH/3] (solid circles). (B) Viability of infected PC346C cellular monolayers 7 days postinfection. Cells were incubated with 1000, 100 or 10 multiplicities of infection (Ad[mock] only MOI 1000) and a viability test (XTT) was performed after 7 days. Each value represents the mean±SD of three or four separate experiments.