Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Jul 11;11(13):4325–4333. doi: 10.1093/nar/11.13.4325

Common terminal repeats of the macronuclear DNA are absent from the micronuclear DNA in hypotrichous ciliate, Stylonychia pustulata.

Y Oka, T Honjo
PMCID: PMC326049  PMID: 6306585

Abstract

Comparison of nucleotide sequences of a macronuclear DNA and its micronuclear counterpart of a hypotrichous ciliate, Stylonychia pustulata, demonstrates that common terminal repeats (C4A4) of the macronuclear DNA are not present at the corresponding region in the micronuclear genome. The results indicate that the common terminal C4A4 repeat is added or translocated during or after the rearrangement of the micronuclear DNA to the macronuclear DNA.

Full text

PDF
4325

Images in this article

4328Image
on p.4328

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ammermann D., Steinbrück G., von Berger L., Hennig W. The development of the macronucleus in the ciliated protozoan Stylonychia mytilus. Chromosoma. 1974 May 10;45(4):401–429. doi: 10.1007/BF00283386. [DOI] [PubMed] [Google Scholar]
  2. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  3. Blattner F. R., Blechl A. E., Denniston-Thompson K., Faber H. E., Richards J. E., Slightom J. L., Tucker P. W., Smithies O. Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections. Science. 1978 Dec 22;202(4374):1279–1284. doi: 10.1126/science.725603. [DOI] [PubMed] [Google Scholar]
  4. Boswell R. E., Klobutcher L. A., Prescott D. M. Inverted terminal repeats are added to genes during macronuclear development in Oxytricha nova. Proc Natl Acad Sci U S A. 1982 May;79(10):3255–3259. doi: 10.1073/pnas.79.10.3255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dawson D., Herrick G. Micronuclear DNA sequences of Oxytricha fallax homologous to the macronuclear inverted terminal repeat. Nucleic Acids Res. 1982 May 11;10(9):2911–2924. doi: 10.1093/nar/10.9.2911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jeffreys A. J., Flavell R. A. A physical map of the DNA regions flanking the rabbit beta-globin gene. Cell. 1977 Oct;12(2):429–439. doi: 10.1016/0092-8674(77)90119-2. [DOI] [PubMed] [Google Scholar]
  7. King B. O., Yao M. C. Tandemly repeated hexanucleotide at Tetrahymena rDNA free end is generated from a single copy during development. Cell. 1982 Nov;31(1):177–182. doi: 10.1016/0092-8674(82)90417-2. [DOI] [PubMed] [Google Scholar]
  8. Klobutcher L. A., Swanton M. T., Donini P., Prescott D. M. All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3' terminus. Proc Natl Acad Sci U S A. 1981 May;78(5):3015–3019. doi: 10.1073/pnas.78.5.3015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lauth M. R., Spear B. B., Heumann J., Prescott D. M. DNA of ciliated protozoa: DNA sequence diminution during macronuclear development of Oxytricha. Cell. 1976 Jan;7(1):67–74. doi: 10.1016/0092-8674(76)90256-7. [DOI] [PubMed] [Google Scholar]
  10. Leder P., Tiemeier D., Enquist L. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system. Science. 1977 Apr 8;196(4286):175–177. doi: 10.1126/science.322278. [DOI] [PubMed] [Google Scholar]
  11. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McTavish C., Sommerville J. Macronuclear DNA organization and transcription in Paramecium primaurelia. Chromosoma. 1980;78(2):147–164. doi: 10.1007/BF00328389. [DOI] [PubMed] [Google Scholar]
  13. Oka Y., Shiota S., Nakai S., Nishida Y., Okubo S. Inverted terminal repeat sequence in the macronuclear DNA of Stylonychia pustulata. Gene. 1980 Sep;10(4):301–306. doi: 10.1016/0378-1119(80)90150-x. [DOI] [PubMed] [Google Scholar]
  14. Prescott D. M., Murti K. G. Chromosome structure in ciliated protozoans. Cold Spring Harb Symp Quant Biol. 1974;38:609–618. doi: 10.1101/sqb.1974.038.01.065. [DOI] [PubMed] [Google Scholar]
  15. Rae P. M., Spear B. B. Macronuclear DNA of the hypotrichous ciliate Oxytricha fallax. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4992–4996. doi: 10.1073/pnas.75.10.4992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Swanton M. T., Heumann J. M., Prescott D. M. Gene-sized DNA molecules of the macronuclei in three species of hypotrichs: size distributions and absence of nicks. DNA of ciliated protozoa. VIII. Chromosoma. 1980;77(2):217–227. doi: 10.1007/BF00329546. [DOI] [PubMed] [Google Scholar]
  18. Szostak J. W., Blackburn E. H. Cloning yeast telomeres on linear plasmid vectors. Cell. 1982 May;29(1):245–255. doi: 10.1016/0092-8674(82)90109-x. [DOI] [PubMed] [Google Scholar]
  19. Tiemeier D. C., Tilghman S. M., Leder P. Purification and cloning of a mouse ribosomal gene fragment in coliphage lambda. Gene. 1977;2(3-4):173–191. doi: 10.1016/0378-1119(77)90016-6. [DOI] [PubMed] [Google Scholar]
  20. Wesley R. D. Inverted repetitious sequences in the macronuclear DNA of hypotrichous ciliates. Proc Natl Acad Sci U S A. 1975 Feb;72(2):678–682. doi: 10.1073/pnas.72.2.678. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES