Table 1. Statistical analysis of 16S rRNA gene clone libraries derived from rhizosphere samples of ‘Ca. L asiaticus'-infected or healthy citrus.
Libraries infected |
Healthy |
Bulk | |||||
---|---|---|---|---|---|---|---|
IT1 | IT2 | IT3 | HT1 | HT2 | HT3 | ||
Accession numbers | JF416062–JF416236 | JF437361– JF437535 | JN052217–JN052392 | JF345348–JF345521 | JN052393–JN052567 | JN052568–JN052739 | JN161828– JN162004 |
Statistics | |||||||
No. of sequences | 175 | 175 | 176 | 174 | 175 | 172 | 177 |
Library coveragea | 81.2 | 87.6 | 76.2 | 77.2 | 78.3 | 76.6 | 89.3 |
No. of OTUsb | 71 | 65 | 84 | 96 | 96 | 95 | 54 |
Diversity indexes | |||||||
Chao1 | 221.1 | 186.4 | 264.3 | 441.23 | 569.2 | 532.2 | 143.2 |
Shannon–Weiner diversity index | 3.8 | 3.7 | 3.8 | 4.3 | 4.2 | 4.3 | 3.5 |
Abbreviations: ‘Ca. L. asiaticus', Candidatus Liberibacter asiaticus; OTUs, operational taxonomic units.
CX=(1−(nx/N)) × 100, where nx is the number of singletons that are encountered only once in a library and N the total number of clones.
OTUs were defined at 97% sequence identity.