Figure 2.

Timing nuclear entry of the SV40 genome. Nuclei of SV40-infected COS-1 cells (at a moi 50) were collected at the indicated time points after infection. The cells were washed and nuclei were isolated as described in Materials and Methods. The nuclear pellet was sonicated and the amount of SV40 DNA was determined by quantitative real-time PCR with SV40-specific primers. SV40 DNA served as a known standard for quantification.