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BAM images of an interfacial meibum film at 24°C at low surface pressure, 3.7 mN/m, (a) and high, 34 mN/m (b). The same meibum film at 35°C is shown at the same levels of compression, although the resultant surface pressures were 2 mN/m (c) and 15 mN/m (d). The mean molecular area of panel c is equivalent to panel a while the film in panel d is at equivalent compression to panel b. The scale bars are 250 μm.
