Figure 2.
Analysis of the initial rate of the transcription reaction. (A) Experimentally measured initial rate of RNA at the various concentrations of each NTP (0.0, 0.4, 0.8, 1.6, 3.2, and 4.0 mM) and Mg(OAc)2 (2 mM; solid circles, 4 mM; open circles, 8 mM; solid triangles, 14 mM; open triangles, and 20 mM; solid squares) concentrations. The RNA concentration was measured after 5-min incubation, and the initial rate was obtained by dividing the concentration by 5. (Error bars) Standard deviation of triplicate experiments. (B) Fitting result of predicted initial rates of RNA to measured ones obtained in panel A. Predicted initial rate was calculated using Eq. 8, and a nonlinear least-square method was used (R2 = 0.96).