(A) Time course of RNA and PPi simultaneously measured under standard conditions with/without PPase. The time points were 0, 10, 20, 30, 40, 50, and 60 min. (Error bars) Standard deviation of triplicate experiments. (B) Time course of RNA, PPi, Mg, and turbidity simultaneously measured under standard conditions. The Mg concentration is plotted on the secondary y axis. The time points were 0, 5, 10, 15, 20, 25, 30, 35, 40, and 45 min. (Error bars) Standard deviation of quadruplicate experiments.