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. 2012 Jan 18;102(2):360–368. doi: 10.1016/j.bpj.2011.12.027

Figure 2.

Figure 2

Matching sequence of a Raman image (by integration over Raman intensities, 80–3040 cm−1) and the corresponding IF data (RGB image). Panel 0 (top row, left) shows the integrated Raman, and panel 8 (bottom row, right) shows the IF image. Panels 1–7 show stepwise overlays demonstrating the quality of image registration achieved by successively changing the respective image contribution. For the registration procedure, the first three (rearranged) PCA components were considered.