Figure 4.

Comparison of aIF images of living (a) and fixed (c) LN18 cells with the corresponding IF image (b). The green channel represents the Golgi apparatus. The overall shape of the cell (cytoskeleton) and the bulk distribution of the specific organelles (Golgi apparatus) are preserved between Raman scanning before and after fixation. The positions of the nucleus and nucleoli in both aIF images are almost unchanged. However, what is probably a fixation artifact can be observed in b and c, affecting the cell in the center. The Raman recording in panel a covers a smaller area compared with b and c. The frame indicates the common part of all images.