Figure 5.

Comparison of original IF images (a–d) and (Raman-based) aIF images (e–h). Generally, the images show high similarities. However, the red channel corresponding to the cytoskeleton differs in that panel a shows high localization, and panel e reveals that actin is actually present throughout the cells. Thus, the correlation between a and e is only 0.36. Another obvious difference is observed between c and g (blue channel): In the IF image the nucleus is inhomogeneously stained, whereas the aIF image reveals the presence of nucleoli at these sites. Yet the correlation is 0.74. (b and f) The images of the Golgi apparatus (green channel) yield a correlation of 0.71. Thus, the RGB overlays in d and h are in great agreement.