Figure 1.

Wound-induced bioluminescence correlates with singlet oxygen production. (A) Luminescence images of Arabidopsis leaves treated with water or the photosensitizer, Rose Bengal. Leaves were vacuum-infiltrated with water or 10 µM Rose Bengal (4,5,6,7-tetrachloro-2′,4′,5′,7′-tetraiodofluorescein) and illuminated for 5 min at a PAR intensity of 750 µmolquanta m⁻² sec⁻¹. (B and C) Luminescence images of wounded Arabidopsis leaves pre-treated with either water (H₂O), 10 mM L-histidine (His) or deuterium oxide (D₂O). Images were taken 5 (A and C) or 10 (B) min after the respective treatments, and are 5 min exposures captured using a fibre optic-coupled CCD system as described previously (Flor-Henry, 2004).⁴