Figure 2.

Wound-induced ROS are derived from photosynthetic electron transport. (A) Histochemical localization of superoxide using NBT under ambient light or in the dark. (B) Histochemical localization of wound-induced hydrogen peroxide using DAB at different light intensities. Photon flux densities determined at bench height are indicated below each leaf (PAR; photosynthetically active radiation). (C) Histochemical localisation of superoxide in the presence or absence (Control) of 10 µM DCMU or DBMIB. (D–F) Light micrographs of untreated Arabidopsis leaf tissue (D), and areas surrounding wound sites in leaves cleared of chlorophyll following staining with NBT (E) or DAB (F).