Figure 2.

Rapid regulation of PIN genes by auxin in the heterozygous abp1/ABP1 receptor mutant and the PIN2 mutant eir1. (A–D) Quantification by real time PCR of transcription in wild-type Ws (filled squares) and abp1/ABP1 seedlings (open squares). (E–H) Quantification by real time PCR of transcription in wild-type Col (filled diamonds) and eir1 seedlings (open diamonds). Methods were described in reference ¹⁶. Two biological treatments with three technical repeats each were used for calculation of average and SD. List of primers: PIN1-forw: GGA GAC TTA AGT AGG AGC TCA GCA; PIN 1-rev: CCA AAA GAG GAA ACA CGA ATG; PIN 2-forw: TAT CAA CAC TGC CTA ACA CG; PIN 2-rev: GAA GAG ATC ATT GAT GAG GC; PIN 3-forw: GAG TTA CCC GAA CCT AAT CA; PIN 3-rev: TTA CTG CGT GTC GCT ATA GT; PIN5-forw: ACC CTG CCG CTC TTC ACC A; PIN5-rev: GCC CAC AAC GCT AAG ACC G.