Figure 1.

Schematic representation of various PIF1 deletion or point mutant constructs used to assess light-induced phosphorylation and degradation. These constructs were expressed as fusion proteins with either Luciferase (LUC) (for full-length PIF1, PIF1-N150, PIF1-C327, PIF1-3M, PIF1-mbHLH) or tandem affinity purification (TAP) (for full-length PIF1 and PIF1-mCK2) tags in transgenic plants. ± indicates the presence and absence of light-induced phosphorylation and degradation, respectively.¹²,¹⁷ The number of + indicates the degree of light-induced phosphorylation and degradation. X indicates the site(s) mutated. n.d., not done yet.