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. 2011 Dec 7;2:244. doi: 10.3389/fmicb.2011.00244

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Copyright © 2011 Nayak, Sarkar, Perrin, Moghimi, Hoffman, Zhou, Byrne and Herzog.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Comparison of Rapa/IL-10/specific peptide tolerization protocol using different routes of drug administration. DO.11.10-tg Rag^−/− mice (n = 4/group) were treated for 3 weeks with Rapa/IL-10/ova-specific peptide, after which the spleens were harvested, and flow cytometry was used to determine: (A) percentage of CD4⁺ T cells of total gated splenocytes and (B) percentage of CD25⁺FoxP3⁺ cells of CD4⁺ T cells. Statistically significant differences between two groups are indicated as *P < 0.05 and **P < 0.01.