Table 1.
Time (min) | Entrapment (%) |
||
---|---|---|---|
pH 7.5 | pH 8.5 | pH 10 | |
0 | 5 ± 0.73 | 75 ± 0.95 | 14 ± 0.97 |
60 | 1 ± 1.82 | 56 ± 1.34 | 13 ± 0.24 |
180 | <1 | 43 ± 1.57 | 12 ± 0.60 |
Content release was measured at various times after removal of nonentrapped solutes by column chromatography (see Methods). 100% release was measured after addition of 1 mM Triton X-100. Values are expressed as the mean ± SD (n = 3).