Figure 1.
Bond rupture follows a complex kinetic behavior that can be robustly represented by the fraction of bonds surviving 1 s after formation. The figure shows a typical survival plot of bonds formed between microspheres coated with 1G4 TCR and surfaces coated with ESO-9V pMHC complex. Wall shear rate was 20 s−1. (A) Vertical bar length is twice the standard error calculated on the basis of binomial law (162 binding events at time zero). (Straight line) Least-square linear fit calculated on [0 s,1 s] time interval. (B) Enlarged initial part of the curve. The slope of the linear fit (straight line), fraction of surviving bonds at 1 s (B1s), and median bond lifetime (t1/2) are shown.