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. 2011 Dec;179(6):2920–2930. doi: 10.1016/j.ajpath.2011.08.022

Figure 1.

Figure 1

BMP-4 signals are transduced in diffuse-type gastric carcinoma cells. A: Expression of BMP and TGF-β signal components in OCUM-12 cells, HSC-39 cells, and OCUM-2MLN cells was analyzed by semiquantitative RT-PCR. Human umbilical vein endothelial cells (HUVECs) were used as positive control for ACVRL1. Two alternatively spliced forms of BMPR2 were detected: the wild-type form (WT) and a short form (SH). B: Diffuse-type gastric carcinoma cells were treated with BMP-4, BMP-6, BMP-9, TGF-β1, or dorsomorphin for 1 hour. Cell lysates were subjected to immunoblotting with antibodies, as indicated under Materials and Methods. C: Diffuse-type gastric carcinoma cells were treated with BMP-4 or dorsomorphin for 2 hours. Expression of ID3 mRNA was determined by quantitative real-time RT-PCR. Data are presented as means ± SD.