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. 2011 Dec;179(6):2920–2930. doi: 10.1016/j.ajpath.2011.08.022

Figure 4.

Figure 4

BMP-4 regulates the expression of CDKN1A in OCUM-12 cells through the SMAD pathway. A: Diffuse-type gastric carcinoma cells were treated with BMP-4 for 1 to 24 hours. Expression of CDKN1A mRNA was determined by quantitative real-time RT-PCR; data are presented as fold change under BMP-4 stimulation (means ± SD). B: Diffuse-type gastric carcinoma cells expressing GFP or dnALK3 were treated with BMP-4 for 24 hours (OCUM-12) or 72 hours (HSC-39). Cell lysates were subjected to immunoblotting with antibodies, as indicated under Materials and Methods. Note ppRB and pRB (arrows). C: OCUM-12 cells were transfected with either siRNA/NTC or siRNA/SMAD4 and then were treated with BMP-4 for 24 hours. Expression levels of SMAD4 mRNA and CDKN1A mRNA were determined by quantitative real-time RT-PCR. Data are presented as means ± SD. D: The cell lysates in C were subjected to immunoblotting with antibodies, as indicated under Materials and Methods.