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. 2011 Dec;179(6):2920–2930. doi: 10.1016/j.ajpath.2011.08.022

Figure 6.

Figure 6

The expression of caALK3 inhibits growth of diffuse-type gastric carcinoma cells in vivo. A: OCUM-2MLN cells were infected with lentivirus carrying GFP cDNA (OCUM-2MLN-GFP) or FLAG-tagged caALK3 cDNA (OCUM-2MLN-caALK3). Cell lysates were subjected to immunoblotting with antibodies, as indicated under Materials and Methods. B: Infected OCUM-2MLN cells were treated with BMP-4 for 1 hour. Cell lysates were subjected to immunoblotting with antibodies, as indicated under Materials and Methods. C: The expression levels of ID3 and CDKN1A mRNA in infected OCUM-2MLN cells were determined by quantitative real-time RT-PCR. Data are presented as means ± SD. D: Infected OCUM-2MLN cells were subcutaneously xenografted into BALB/c nu/nu mice (OCUM-2MLN-GFP, n = 7; OCUM-2MLN-caALK3, n = 8). Quantification of tumor volume over time (day 0 to day 32) are shown. Data are presented as means ± SE. *P < 0.05. E: Schematic representation of BMP signaling pathway negatively regulating proliferation of diffuse-type gastric carcinoma cells via induction of p21 through the SMAD pathway.