Figure 2.

The inflammatory pathway is the most affected among the up-regulated genes at 24 hours after UVB exposure. A: The relationships among significantly up-regulated genes involved in inflammatory response pathways in Ogg1 knockout mice at 24 hours after UVB exposure. Significantly up-regulated genes, previously shown to be involved in skin, were selected from microarray data (red rectangles). The schematic figure was modified from WikiPathways (available at http://www.wikipathways.org/index.php/Pathway:WP458). B: Relative IL-1β gene expression levels in wild-type (WT) and Ogg1 knockout (KO) mouse skin, as determined by real-time quantitative PCR. Each of the two groups of RNAs isolated from wild-type and Ogg1 knockout mice was assayed in duplicate. **P < 0.01. Data are representative of three separate determinations. C: IL-1β gene expression levels in the skin of wild-type and Ogg1 knockout mice at 24 hours after UVB exposure. Positive signals are seen as reddish-brown deposits produced on reaction with the 3-amino-9-ethylcarbazole substrate. The UVB dose was 250 mJ/cm². Weak or no expression of IL-1β is seen in wild-type mouse skin with and without UVB exposure. Scale bar = 30 μm.