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. 2011 Dec;179(6):2810–2821. doi: 10.1016/j.ajpath.2011.08.023

Figure 1.

Figure 1

Characteristics of GLO1-overexpressing aged rats and amelioration of senescence-associated renal dysfunction and urinary protein excretion. No significant differences were observed between young and aged WT and Tg-GLO-1 rats for random (nonfasting) blood glucose levels (A), for levels of GSH, an essential cofactor of GLO1, in renal cortex lysate (data adjusted by protein concentration) (F), for total SOD activity of renal cortex lysate (data adjusted by protein concentration) (G), and for relative transcript levels of Mn-SOD (determined by real-time quantitative RT-PCR, with the value for young WT rats set as 1) (H). No significant differences were observed between aged WT and Tg-GLO-1 rats for fasting blood glucose levels (B), for fasting blood insulin levels (C), for HOMA-IR (calculated by multiplying fasting blood glucose and fasting blood insulin, then dividing by 405) (D), and for blood HbA1c levels (E). I: GLO1 activity of renal cortex lysate of WT animals decreased in an age-dependent manner. Data were adjusted by protein concentration. *P < 0.01. J: GLO1 activity of renal cortex lysate in Tg-GLO1 rats was markedly higher than that in WT rats, and did not show an age-dependent decrease. Data were adjusted by protein concentration. *P < 0.01 versus young WT rats. K: Distribution of GLO1 in rat kidney, shown in representative micrographs of immunohistochemical staining of GLO1 in the rat renal cortex. GLO1 was expressed ubiquitously in the kidney of both WT and Tg-GLO1 rats. Methyl Carnoy's fixed specimens (4 μm thick) were counterstained with hematoxylin. Original magnification = ×400. Scale bars: 50 μm. L: Serum creatinine levels determined by enzymatic assay indicate that the age-dependent deterioration of renal function was ameliorated by GLO1 overexpression. *P < 0.01; **P < 0.05. M: Serum cystatin C levels indicate that the age-dependent deterioration of renal function was ameliorated by GLO1 overexpression. *P < 0.01. N: An age-dependent increase in proteinuria was prevented by GLO1 overexpression. Urine was collected for 24 hours using metabolic cages. *P < 0.05.