Figure 3.

Immunohistochemical analysis of HSCs and macrophage recruitment after CCl₄ administration. Liver sections from DDR2^−/− and DDR2^+/+ mice treated with CCl₄ for 10 or 30 days were stained with anti-desmin, anti–α-SMA, or anti-F4/80 antibodies. A and B: Computer-assisted semiquantitation of stained cells within the fibrotic scar in microphotographed labeled sections. Data are expressed as the percentage of region of interest, which was restricted to scarring tissue C: Semiquantitation of anti-F4/80–stained macrophages associated with the scar. Only F4/80-positive cells located within or opposed the scar tracts were counted. *P < 0.05, significantly different compared with the DDR2^+/+ group. Lower panels:DDR2^+/+ group. Lower panels: Microphotographs of liver sections from DDR2^+/+ and DDR2^-/- mice treated with CCL₄ for 30 days and stained with anti-desmin (red; A), anti–α-SMA (blue; B) and anti-F4/80 (red; C) antibodies.