Figure 4.

DDR2 is induced in stellate cells during chronic CCl₄ injury. Immunohistochemical detection of DDR2 expression in mouse liver. DDR2 expression (red) was analyzed in untreated livers (A) after 10 days (B) or after 30 days (C) of CCl₄ treatment. D and E: β-galactosidase activity (blue) in DDR2 knock-out/LacZ knock-in mice untreated (D) or treated with CCl₄ for 30 days (E). F: Masson staining of DDR2 knock-out/LacZ knock-in mice treated with CCl₄ for 30 days. G: Western blot of DDR2 expression and phosphorylation in DDR2^−/− and DDR2^+/+ stellate cells freshly isolated from untreated livers, or treated with CCl₄ for 10 and 30 days. CV, central vein; IB, immunoblot; IP, immunoprecipitation; PT, portal tract.