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. 2011 Dec;179(6):2894–2904. doi: 10.1016/j.ajpath.2011.09.002

Figure 6.

Figure 6

Profibrotic role of macrophages from chronically injured DDR2−/− livers. A: Quiescent HSCs from oil-treated and activated HSCs from CCl4-intoxicated livers were allowed to migrate for 16 hours through collagen type I–coated porous upper wells of migration chambers in response to media conditioned by macrophages from fibrotic livers (MPH-CM). B: Gelatinase and collagenase activities in supernatants from macrophages isolated from fibrotic livers and cultured for 16 hours. C: Differential mRNA expression pattern of fibrosis-related genes in macrophages isolated from fibrotic livers. (n = 16 mice from 2 independent treatments, 2 mice per group). Relative mRNA expression in macrophages from control DDR2+/+ livers compared to RPL13 mRNA expression: TGF-β: 0.06 ± 0.007; tumor necrosis factor-α (TNF-α): 1.61 ± 0.254; IL-6: 0.08 ± 0.002; monocyte chemoattractant protein-1 (MCP-1): 0.31 ± 0.034. *P < 0.05 relative to DDR2+/+ HSC in DDR2+/+ MPH-CM (A) or control DDR2+/+ livers (B and C).